General requirements
In general, the layout plan of a spawn laboratory should have a total built up area of 19x8x3.6 m (LxBxH). This area will be divided into different work areas like cooking/autoclaving room, inoculation room, and incubation room, washing area, store, office and one cold storage. Cold storage room of 3x3 x3.6 m (LxBxH) is enough to store the spawn at 4-5°C. The walls, roof floor as well as door is provided with heavy insulation (7.5-10 cm thickness) and two air conditioner (each of 1.5 tonnes capacity) are required to maintain the temperature inside the room. Incubation room 2 (3x6.0x3.6 m, LxBxH) with entire surface area (wall, floor, ceiling, doors) insulated with 5-7.5 cm thick insulation is required. Two air conditioners (each 1.5 tonnes capacity) are required for maintenance of temperature (25°C) in the incubation room. Besides these, some ancillary structures like office, small lab space, delivery area etc. may also be required.
The equipment and other miscellaneous items required in a spawn laboratory are:

Big Autoclave (Horizontal type): It is required for the sterilization of grain bottles and substrates filled in polypropylene bags for producing spawn and also the non-composted substrates for production of specialty mushrooms.

Small Autoclave (Standing type): It is for the sterilization of culture media in tubes / flasks and the substrates, including grains for production of Master culture and spawn in glass bottles / PP bags on a small scale.

Pressure Cooker: A big size pressure cooker (5-10 litre capacity) will be required for    sterilization of media for routine laboratory work.

Boiler: Baby boiler run by wood fuel, electricity or diesel will be required for production of   pressure steam for boiling, sterilization of grains and pasteurization of casing mixture.

Laminar Flow: It is required for isolation of fungi and inoculation of grain bags / bottles with master cultures under aseptic conditions.

Weighing Machine: Weighing machines is necessarily required for the exact measurement of raw materials for producing spawn and compost.

Steel or cemented racks: Racks are required in the incubation and storage rooms on which the inoculated bags are to be kept at a particular temperature for mycelial run and their storage at different temperatures.

Steel Trolleys: About 5-6 pushing type steel trolleys will be required for easy movement and carriage of grain bags, spawn bottles, compost bags and other materials from one room to another room.

BOD Incubators: These are required to incubate cultures inoculated or transferred in tubes, Petri dishes, flasks and Master culture bottles for their speedy growth at a fixed temperature.

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