Mushroom spawn production technology

Durga Prasad1, R. P. Singh2 and S.K. Gangwar3
1College of Agriculture, Baytu-344034, Barmer, Agriculture University, Jodhpur, Rajasthan
2Senior Scientist and Head, KVK, West Champaran-II, Dr RPCAU, Pusa, Samatipur, Bihar
3Senior Scientist and Head, KVK, West Champaran-I (Dr RPCAU, Pusa, Samatipur, Bihar)
1Corresponding author’s email: dp.coabaytu@gmail.com
Posted on October 15, 2022


General requirements
In general, the layout plan of a spawn laboratory should have a total built up area of 19x8x3.6 m (LxBxH). This area will be divided into different work areas like cooking/autoclaving room, inoculation room, and incubation room, washing area, store, office and one cold storage. Cold storage room of 3x3 x3.6 m (LxBxH) is enough to store the spawn at 4-5°C. The walls, roof floor as well as door is provided with heavy insulation (7.5-10 cm thickness) and two air conditioner (each of 1.5 tonnes capacity) are required to maintain the temperature inside the room. Incubation room 2 (3x6.0x3.6 m, LxBxH) with entire surface area (wall, floor, ceiling, doors) insulated with 5-7.5 cm thick insulation is required. Two air conditioners (each 1.5 tonnes capacity) are required for maintenance of temperature (25°C) in the incubation room. Besides these, some ancillary structures like office, small lab space, delivery area etc. may also be required.
The equipment and other miscellaneous items required in a spawn laboratory are:

Big Autoclave (Horizontal type): It is required for the sterilization of grain bottles and substrates filled in polypropylene bags for producing spawn and also the non-composted substrates for production of specialty mushrooms.

Small Autoclave (Standing type): It is for the sterilization of culture media in tubes / flasks and the substrates, including grains for production of Master culture and spawn in glass bottles / PP bags on a small scale.

Pressure Cooker: A big size pressure cooker (5-10 litre capacity) will be required for    sterilization of media for routine laboratory work.

Boiler: Baby boiler run by wood fuel, electricity or diesel will be required for production of   pressure steam for boiling, sterilization of grains and pasteurization of casing mixture.

Laminar Flow: It is required for isolation of fungi and inoculation of grain bags / bottles with master cultures under aseptic conditions.

Weighing Machine: Weighing machines is necessarily required for the exact measurement of raw materials for producing spawn and compost.

Steel or cemented racks: Racks are required in the incubation and storage rooms on which the inoculated bags are to be kept at a particular temperature for mycelial run and their storage at different temperatures.

Steel Trolleys: About 5-6 pushing type steel trolleys will be required for easy movement and carriage of grain bags, spawn bottles, compost bags and other materials from one room to another room.

BOD Incubators: These are required to incubate cultures inoculated or transferred in tubes, Petri dishes, flasks and Master culture bottles for their speedy growth at a fixed temperature.

................see more

For Details Download PDF


  • Ahlawat, O.P. and Rai, R.D. (2000). Mushroom Science XV, 695-699.
  • Aneja, K.R. (2004). Mushroom production technology. In: Experiments in Microbiology Plant Pathology and Biotechnology (Fourth Edition). New Age International Limited, Publishers. New Delhi. pp. 496-519.
  • Chadha, K.L. and Sharma, S.R. (1995). Advances in Horticulture. Volume 13, Mushroom. Malhotra Publishing House, New Delhi, 649 p.
  • Chang, S.T. (2006). The world mushroom industry: trends and technological developments. Int J Med Mushrooms. 8: 297-314.
  • https://nrcmushroom.org/html/technicalbulletin.html
  • https://www.agrimoon.com/
  • Singh, M., Vijay, B., Kamal, S., Wakchaure, G.C. (2011). Mushrooms: cultivation, marketing and consumption. Directorate of Mushroom Research. ICAR-Chambaghat, Solan –173213 (HP), 266pp
  • Upadhyay, R.C., Singh, S.K., and Tewari, R.P. (2004). Mushroom spawn production and infrastructure requirements. Technical Bulletin, DMR, Solan. pp. 38.

Note Society for Science and Nature(SFSN) do not hold any responsibility about the views, opinion and work of author(s) presented in the article published in SFSN